Releases nucleic acids from fresh, dried or frozen whole blood samples

  • Simple 2-pipette step protocol reduces consumable costs and incubation times

  • Samples prepared in less than three minutes with no centrifugation, heat, or enzymes.

  • Stabilises DNA at ambient temperature

  • Sequesters PCR inhibitors

  • Facilitates automation

Arcis Blood Kit

UFL005 50 Preps £312
UFL011 500 Preps £POA

 

Need

  • Extraction efficiency that ensures adequate yield
  • Nucleic acid stabilisation without need for cold chain
  • Suppress PCR inhibitor activity
  • Ability to adapt to variable sample volumes without adding time-to-result
  • Safe handling for laboratory personnel

Solution

  • Rapid cell lysis maximises yield of nucleic acid extraction
  • Stabilises DNA for up to 90 days at ambient temperature
  • Sequesters PCR inhibitors
  • Simplified, scalable protocol to process dilute samples or increase sensitivity
  • REACH-compatible chemistry removes safety risks

Use Arcis Blood Prep Kit to enable nucleic acid extraction from dried, fresh or frozen whole blood samples. The nucleic acids released by the kit have been successfully used directly in molecular biology techniques including qPCR, Next Generation Sequencing and Isothermal Amplification without the need for further clean-up or purification steps.

Standard Protocol

If samples are frozen ensure that they have thawed completely before starting this procedure.

  1. Add 30μl of sample to 150μl of Reagent 1 (or scale up for larger sample volume). Mix thoroughly using a pipette or by vortex mixing.
  2. Incubate for one minute at room temperature. At this point DNA is stabilised for 90 days and RNA is stabilised for up to 7 days at room temperature, provided there is no further processing. The sample may show haem carry over and therefore be red in colour but this will not interfere with subsequent processing.
  3. Take 5μl of the above lysed mixture and combine with 20μl of Reagent 2 (or scale up for larger sample volumes maintaining the 1:4 ratio). Once processed with Reagent 2, samples should be used within 4 hours or frozen at -20˚C.
  4. Add appropriate volume into PCR master mix (e.g. 5μl per 25μl reaction) or continue directly to other downstream technique.

Dilute Sample /Enhanced Sensitivity Protocol

For applications where enhanced sensitivity is required, or when handling very dilute research samples such as serum or plasma, the ratio of sample to Arcis Reagent 1 can be increased to avoid further dilution.

The following protocol modifications can be used:

  1. Samples can be mixed with Reagent 1 at a 1:4 or 1:3 ratio to reduce sample dilution (See Table 1).
  2. Samples that have been processed in step 6.1 can be added to Reagent 2 at 1:3, 1:2 or 1:1 ratio to reduce sample dilution (See Table 2).

Table 1: Processing samples in Reagent 1*

Sample Volume (μl) Reagent 1 Volume (μl) Ratio
30 120 1:4
30 90 1:3
60 180 1:3

Table 2: Reaction mixture samples in Reagent 2

Extract from lysis reaction (μl) Reagent 2 Volume (μl) Ratio
5 15 1:3
10 20 1:2
20 20 1:1

*increasing volume of reagents will reduce the total number of samples that can be processed by the kit.

Arcis Sample Prep Technology

Arcis sample prep technology is a fast and convenient 2-step protocol for preparation of nucleic acids from a variety of sample types. The nucleic acids are prepared and stabilised in 3 minutes without the need for instrumentation or complicated heating or cooling steps. The DNA and RNA extracted is suitable for direct use in PCR, qPCR and RT-qPCR reactions using standard and fast cycling protocols.

  • Simple 2-step process
  • No instrumentation required
  • Nucleic acids stabilised after 1 minute – DNA shown to be stable in Reagent 1 for up to 90 days
  • Shipped and stored at ambient temperature

Step 1

Reagent 1:

Opens up cells and releases nucleic acids in less than 1 minute. At this point the nucleic acids are released and protected by the Arcis solution, but are not in a usable state. DNA in Reagent 1 is stable for up to 90 days at room temperature.

Step 2

Reagent 2:

Binds inhibitors and enhances PCR while also relaxing the DNA into a usable confirmation.  At this point the sample should be used immediately, going straight into PCR, qPCR, RT-qPCR or sequencing.

Relaxation of DNA:

The Arcis Sample Prep system consists of 2-steps. In the first step the sample is combined with a chemical which results in powerful and controlled lysis of cells and tissue. The first step also simultaneously chelates the nucleic acid and stabilises DNA for up to 90 days at room temperature. The critical second step contains a proprietary buffer which removes the nucleic acid chelation and relaxes the DNA/RNA in preparation for PCR or RT-qPCR. The proprietary buffer has also been formulated to mop up any PCR inhibitors that may have been carried over from the first step.

Validation Data:

The Arcis Sample Prep kits were compared to market leading kits and evaluated for speed and yield. The Arcis DNA Sample Prep Kits required no instrumentation and took less than three minutes from start to finish. In contrast all the other kits required centrifugation or heating and frequently required a separate lysis step before the protocol. The processing times of the kits ranged from 20 minutes to 4 hours. The total DNA extracted from each kit was assessed using the Qubit System. The P kit yielded the most DNA, but was labour intensive and time consuming kit. The Arcis kit was comparable to the Q kit, however DNA was obtained in 3 minutes compared to 60 minutes for the Q system.

The Arcis Blood Prep Kit is a ready to use kit comprising two pre-filled reagent tubes enabling extraction of nucleic acids from blood samples prior to a flexible range of downstream assays. The product is intended to be used by trained users proficient in molecular biological techniques.

Examples of applications:

Product code

UFL005, UFL011

Sample type

Blood (fresh, dried or frozen whole blood)

Sample input volume

Varies

Reaction time

Varies

No. of reactions

50, 500

Storage

Store at room temperature, do not freeze

Shipping conditions

Room temperature