DNA and RNA extraction from eukaryotic, prokaryotic cells and viruses
Simple 2-pipette step protocol reduces consumable costs and incubation times
From sample to detection in under 3 minutes
Ideal for austere environments by avoiding centrifugation, heating, and enzymatic treatments
Versatile for use in biothreat detection and other applications
Non-hazardous chemistry
- Extraction efficiency that ensures adequate yield
- Nucleic acid stabilisation without need for cold chain
- Ability to adapt to variable sample volumes without adding time-to-result
- Safe handling for laboratory personnel[
Solution
- Rapid cell lysis maximises yield of nucleic acid extraction
- Stabilises DNA for up to 90 days at ambient temperature
- Scalable protocol to process wide range of sample volumes
- REACH-compatible chemistry removes safety risks
Standard Protocol
1.If samples are frozen ensure that they have thawed completely before starting this procedure. Add 90μl of sample to 150μl of Reagent 1 (or scale up for larger sample volume). Mix thoroughly using a pipette or by vortex mixing.
2.Incubate for one minute at room temperature. At this point DNA is stabilised for 90 days and RNA is stabilised for up to 7 days at room temperature, provided there is no further processing.
3.Take 5μl of the above lysed mixture and combine with 20μl of Reagent 2 (or scale up for larger sample volumes maintaining the 1:4 ratio). Once processed with Reagent 2, samples should be used within 4 hours or frozen at -20 ̊C.
4.Add appropriate volume into PCR master mix (e.g. 5μl per 25μl reaction) or continue directly to other downstream technique.
Dilute Sample Protocol
1.When handling very dilute samples such as saliva the ratio of sample to Arcis Reagent 1 can be increased to 1:1 to avoid further dilution (90μl of sample to 90μl of Reagent 1).
2.Samples that have been processed as in step 1 of the standard protocol can be added to Reagent 2 at 1:3, 1:2 or 1:1 ratio to reduce sample dilution. See Table.
| Extract from lysis reaction (μl) | Reagent 2 (μl) | Ratio |
|---|---|---|
| 5 | 15 | 1:3 |
| 10 | 20 | 1:2 |
| 20 | 20 | 1:1 |
Arcis Sample Prep Technology
Arcis sample prep technology is a fast and convenient 2-step protocol for preparation of nucleic acids from a variety of sample types. The nucleic acids are prepared and stabilised in 3 minutes without the need for instrumentation or complicated heating or cooling steps. The DNA and RNA extracted is suitable for direct use in PCR, qPCR and RT-qPCR reactions using standard and fast cycling protocols.
- Simple 2-step process
- No instrumentation required
- Nucleic acids stabilised after 1 minute – DNA shown to be stable in Reagent 1 for up to 90 days
- Shipped and stored at ambient temperature
Reagent 2:
Binds inhibitors and enhances PCR while also relaxing the DNA into a usable confirmation. At this point the sample should be used immediately, going straight into PCR, qPCR, RT-qPCR or sequencing.
Relaxation of DNA:
The Arcis Sample Prep system consists of 2-steps. In the first step the sample is combined with a chemical which results in powerful and controlled lysis of cells and tissue. The first step also simultaneously chelates the nucleic acid and stabilises DNA for up to 90 days at room temperature. The critical second step contains a proprietary buffer which removes the nucleic acid chelation and relaxes the DNA/RNA in preparation for PCR or RT-qPCR. The proprietary buffer has also been formulated to mop up any PCR inhibitors that may have been carried over from the first step.
Validation Data:
The Arcis Sample Prep kits were compared to market leading kits and evaluated for speed and yield. The Arcis DNA Sample Prep Kits required no instrumentation and took less than three minutes from start to finish. In contrast all the other kits required centrifugation or heating and frequently required a separate lysis step before the protocol. The processing times of the kits ranged from 20 minutes to 4 hours. The total DNA extracted from each kit was assessed using the Qubit System. The P kit yielded the most DNA, but was labour intensive and time consuming kit. The Arcis kit was comparable to the Q kit, however DNA was obtained in 3 minutes compared to 60 minutes for the Q system.
The Arcis Sample Prep Kit is particularly suited to the following areas:
|
Product code |
UFL002, UFL009 |
|
Sample type |
Cells (animal, plant or bacterial) |
|
Sample input volume |
Varies |
|
Reaction time |
Varies |
|
No. of reactions |
50, 500 |
|
Storage |
Store at room temperature, do not freeze |