PCR DIRECT inactivates ≥ 99.99% coronavirus
PCR DIRECT ETM protects RNA for up to 4 days at ambient temperature
PCR DIRECT ETM lyses virus and donor cells, protects released RNA and DNA, and inhibits nucleases at ambient temperatures. To measure RNA stability, heat inactivated virus (BEI) was spiked into raw saliva and tested over four days by RT-qPCR. This stabilized samples can be transported without cold-chain to laboratories, where samples can safely reside within automated liquid handlers while RT-qPCR tests are assembled.
Improve automated liquid handling of saliva by removing viscosity
An automated liquid handler (OT-2, Opentrons) attempts to aspirate saliva that was freshly collected via spitting. Before the addition of PCR DIRECT, long filaments remain attached to the pipette tips. After the addition of PCR DIRECT, the automated pipettors easily aspirate the liquified saliva.
To quantify the impact on accurate pipetting, the volume of aspirated saliva and saliva treated with PCR DIRECT was measured by increasing volumetric aspirations with a micropipettor. This was then converted into percentage of target volume pipetted and in shown in the chart to the right. Without treating raw saliva, there is an approximately 30% reduction in aspirated versus target volume of saliva. This significant reduction in aspirated saliva would result in under sampling and subsequent losses in sensitivity.